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Freeze-Dried Oxidized Alginate–Gelatin Scaffold Coated with Reduced Graphene Oxide for Bone Tissue Engineering

Authors :

Mohsen Aghababaei Tafreshi¹ Sameereh Hashemi-Najafabadi² Nafiseh Baheiraei³

1- دانشگاه تربیت مدرس 2- دانشگاه تربیت مدرس 3- دانشگاه تربیت مدرس

Keywords :

Oxidized Alginate،Schiff Base،Conductive،Bone Tissue

Abstract :

In this study, a three-dimensional scaffold of oxidized alginate-gelatin (OAL-Gel) was successfully fabricated via a Schiff base reaction. To make the scaffold suitable for bone tissue engineering and create a conductive platform, reduced graphene oxide (rGO), a widely-used conductive material, was applied as a coating. Characterization of the materials and scaffolds began by determining the degree of alginate oxidation (DO) using a titration method. The experimental DO was calculated to be 60%, while the theoretical DO was 50%. Fourier Transform Infrared (FTIR) spectroscopy on the final scaffold and oxidized alginate confirmed the presence of aldehyde groups and Schiff base bonds, validating the successful oxidation of alginate and the intended scaffold fabrication. Furthermore, scanning electron microscopy (SEM) images of the different scaffold groups showed that the alginate-gelatin scaffold cross-linked with calcium chloride had a more organized and uniform network. In contrast, OAL-Gel scaffold exhibited less uniformity due to Schiff base bonds. Overall, this study demonstrates that OAL-Gel scaffold coated with rGO has high potential for use in bone tissue engineering due to its conductivity and biocompatibility.